Possible Microorganisms | Description | Investigations |
Escherichia coli(Most common cause of UTI) |
-Belongs to family of Enterobacteriaceae(Escherichia)
-Clinical findings: UTI (Pyelonephritis and Cystitis), diarrhea disease
-Is a member of normal intestinal flora
| -Microscopy and gram staining: Gram negative rod
-Culture: Haemolyic colonies on blood agar, lactose fermenting colonies (Red/Pink) on MacConkey agar, green metallic sheen on EMB agar
-Biochemical tests:
-Indole positive
-Methyl red positive
- Voges-Proskauer (VP) negative
-Urease negative
-Citrate negative
-Catalase positive
-Oxidase negative
-TSI: acidic slant/acidic deep/no H2S
-Susceptibility testing: ampicillin, cephalosporin, aminoglycosides, sulfonamides
|
Staphylococcus saprophyticus(Second most common cause of UTI) |
-Belongs to family of staphylococci
-Clinical findings: Urinary tract infection
-Present in the urinary tract and bladder of sexually active females
| -Microscopy and gram staining: Gram positive cocci in clusters
-Culture: Pale colonies on blood agar
-Biochemical tests:
-Catalase positive
-Coagulase negative
-Susceptibility testing:Quinilone
|
Klebsielle Pneumoniae |
- Belongs to family of Enterobacteriaceae(Klesbsiella)
- Clinical findings: urinary tract infection
-Present in the respiratory tract and feces of about 5% normal individuals
| -Microscopy and gram staining: Gram negative rod
-Culture: Pink, mucoid colonies on MacConkey agar
-Biochemical tests:
-Indole negative
- Methyl red negative
-Voges-Proskauer reaction positive
- Urease negative
-Citrate positive
- TSI: acidic slant/acidic deep/no H2S
-Susceptibility testing:Cephalosporin
|
Enterobacter aerogenes |
- Belongs to family of Enterobacteriaceae(Enterobacter)
- Clinical findings: urinary tract infections and sepsis
- Present in the intestinal tract-
| -Microscopy and gram staining: Gram negative rod
-Culture:Pink to purple colonies on EMB agar
-Biochemical tests:
-Indole negative
-methyl red negative
-VP positive
-urease negative
- Citrate positive
-TSI: acidic slant/acidic deep/no H2S
Susceptibility testing: cephalosporin
|
Particulars of patient
Proteus mirabilis |
- Belongs to family of Enterobacteriaceae(Proteus)
- Clinical findings: urinary tract infections and produce bacteremia, pneumonia
| -Microscopy and gram staining: Gram negative rod
-Culture: Pale colonies on MacConkey agar, swarming growth on blood agar
-Biochemical testing:
-Indole negative
-Methyl red positive
-VP positive/negative
-Urease positive
- Citrate positive
-Oxidase negative
-TSI: alkaline slant/acidic deep/H2S
-Susceptibility testing: penicillin, cephalosporins, quinolones
|
Name:Khong Fay Seah
Sex:Female
Age:27
Clinical Diagnosis
Complaints: fever,chills,dysuria
Diagnosis:urinary tract infection
Antibiotic treatment: if any (nil)
Enterococcus faecalis |
- Belongs to family of Streptococci
-Clinical findings: abdominal abscess, urinary tract infection ((bladder infection, Pyelonephritis)
-Present in colon
| -Microscopy and gram staining: Gram positive cocci in chains
-Culture: Red dot colonies on MacConkey agar
-Biochemical testing:
-Decolourise the litmus milk,
-Indole negative
-Catalase negative
-Susceptibility testing: Vancomycin,ampicillin
|
Pseudonomas aeruginosa |
- Belongs to family of Pseudomonads
-Clinical findings: Typically infects the pulmonary tract, urinary tract(Pyelonephritis)
-Distributed in nature and is commonly present in moist environments in hospital
| -Microscopy and gram staining: Gram positive spherical
-Culture: Greenish pyocyanin pigment on MacConkey agar
-Biochemical testing:
-Indole negative
-Urease negative
-Oxidase positive,
-TSI: allaline slant/alkaline deep
-Susceptibility testing: Ciprofloxacin,imipenam
|
PICTURES OF THE POSSIBLE CAUSATIVE AGENTS
E Coli ( red dot colonies on Mac agar)
K. Pneumonia (red/pink colonies on Mac)
Staphyloccocus Saprophyticus
Pseudomonas aeruginosa
Enterococcus faecalis
Enterobacter aerogenes
Proteus mirabillis
References
http:www.yahoo.com>image search> Proteus mirabilis
http:www.yahoo.com>image search> Enterobacter Aerogenes
http:www.yahoo.com>image search>Enterococcus faecalis
http:www.yahoo.com>image search>Pseudomonas aeruginosa
http:www.yahoo.com>image search>Staphyloccocus Saprophyticus
http:www.yahoo.com>image search>K. Pneumonia
http:www.yahoo.com>image search> E Coli
http://www.mc.maricopa.edu/~johnson/labtools/Dbiochem/emb.html>EMB agar
http://en.wikipedia.org/wiki/MacConkey_agar> MacConkey Agar
http://en.wikipedia.org/wiki/Blood_agar#Blood_agar_types> blood agar
Book
Tony Hart, Paul Shears (2004).Color Atlas of Medical Microbiology: Elsevier’s Health Sciences Department
kai lin
0503211E
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