Follow-up of case 6

Above is the procedure that i will carry out during laboratory to determine the identity of the microoraganism present in the vaginal swap.

Materials Needed:
1) Blood Agar
2) MacConkey Agar
3) Gram stain Reagent
4) Caoagulase Reagent
5) Oxidase Reagent
6) Catalase Reagent
7) Commercial ID Testing Kits
8) Biochemical Tests Reagents
9) Antibody lines
Patient: Ong Fei Fei
Age: 37 Years old
Complaints: Fever, pain during urination, virginal discharge
Diagnosis: UTI

1)Enterococcus Faecalis
Labortatory Diagnosis:
(i) Alpha, beta or non hemolytic colonies on blood agar
(ii) Grow in 6.5% NaCl and hydrolyzes esculin on the presence of 40% bile
(iii)Catalase Negative

2)Eschericahia coli
Labortatory Diagnosis:
(i) Lactose fermenting on macConkey agar
(ii) TSI show acid slant, acid butt with gas but no H2S
(iii)Indole +
(Iv) Motile

3)Enterobacter-Klebsiella-Serrtia Family
Labortatory Diagnosis:
(i) Lactose fermenting on macConkey agar
(ii) Appear as mucoid colonies on agar plate

4)Proteus-Providencia-Morganella Family
Labortatory Diagnosis:
(i) Non-lactose fermentor on Macconkey
(ii) Distinct swarming on blood agar
(iii)P.vulgaris and P.mirabilis produce H2S that blacken the butt of TSI agar
(iv) P.mirabilis is indole negative whereas the other 2 are indole positive
(v) Urease Positive

Antibiotic suceptibility Testing will be carried out on antibodies mention in previous posting after bacterial is identified.

Yeo Ching Wei
0503288C