HELLO!
section posted: flow cytometry lab
Name of test: CD 4 and CD 8 count
Purpose of the test : it is used as a diagnostic and prognostic tool for clinical classification of immunodeficiency states such as autoimmune state and immune monitoring of responses to therapy such as to monitor progression of AIDS by measuring CD4 and CD 8 levels.
CD4 is expressed by T-Helper cells (collaborate with B cells promoting their proliferation and promote t cytotoxic cells development.)
CD8 is expressed by T- suppressor cells (suppress immune responses)
Principle of the test: flow cytometry has been commonly used for the measurement of surface marker antigens by fluorescent labeled monoclonal antibody which will pass through laser beam generating scattered and fluorescent signal that can be processed by computers. These surface makers are related to specific functions and subgroups.
Clinical significance: CD4 and CD 8 measurement are used to monitor progression of AIDS as CD4 cells are depleted by HIV infection whereas CD8 cells persist.
well, that's all! have fun for sip..
kai lin
0503211E
tg02
9 comments:
heyheys MS SIM! (:
what's flow cytometry? and are there any reference range for CD4 and CD 8 levels?
thanks girl! :D
dorothy
TG01
HELLO =)
I m interested to know the ref. range for the CD4/8 levels too!
Chen kangting
0503331A
TG02
hey kailin,
just 2 questions :)
what are the other diseases that require this test and what will the results be like?
phuiyuen
TG02
heya girl...juz wanna ask u y isit that CD4 cells are depleted by HIV infection while CD8 cells persist? and about the fluorescent labeled mAb...what type/examples of mAb are being used?
aniwei njoy ur SIP girl:)
nur zahirah tg02
hey.. juz wondering.. is flow cytometry possible for other conditions such as anemia or leukaemia? If so.. wad markers are used.. (for example.. CD4 and CD8 to differentiate the different types of T cells).. thanks..
Jia Hao
hello~ it's a coincidence that i'm doing the flow cytometry too! It's quite a useful instrument! though boring wen acquiring data. I'm using it for lymphocyte analysis.. borrowed one thick book on flow cyto.. hope to share with u what i'd learnt soon..
Joan
TG01
hiya,
I would like to ask ya what do u mean by"these surface markers are related to specific functions and subgroups". Hmm..can explain what are the specific functions and subgroups?
Thanks ya!
Michelle
Hi kailin,
i'm wondering if conducting this test (measurement of CD4 and CD8 levels) alone is enough to diagnose and give respective treatment to the patient?
Thanks.
Yvonne
TG01
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